Single molecule tracking reports live biophysical properties of molecules being investigated besides their coding sequence. It has evolved as a novel approach complementing genomic sequencing. Here we provided “sojourner” package, to address statistical and bioinformatic needs related to the analysis and comprehension of high throughput single molecule tracking data.
sojourner package provides statistical analysis of biophysical properties of single molecules. Current version primarily uses mean square displacement (MSD) based analysis, more functions using displacement based and hidden Markov model based method will be added in future release.
Current version includes: dwellTime (duration of the tracks), squareDisp (squared displacement), MSD (mean square displacement), Dcoef(diffusion coefficient), CDF(cumulative distribution function), etc., and various plotting functions for visulization. It also includes a graphical user interface (GUI) sojournerGUI(). The GUI version has the most frequently used functions but not all command line ones.